Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet: Neutralizing mAbs as pre-exposure prophylaxis in k18-hACE2 mice CV1-1, CV1-30, and CV2-75 were assessed to see whether they could confer protection in a mouse model. (A) Experimental timeline. (B) Number of PFUs in the lungs 2 days following challenge. Error bars indicate SD. (C) Viral RNA in lung tissue 2 days after challenge was measured by qPCR and normalized to GAPDH expression. Error bars indicate SD. (D) Kaplan-Meyer survival curve of the viral load/titer in the lungs of remaining mice comparing the various treatment groups. Statistics were determined by one-way ANOVA with Dunnett’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Expressing, Comparison

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet:

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Virus, Infection, Recombinant, Affinity Column, Purification, Staining, Cloning, Cell Isolation, Transfection, Luciferase, Reverse Transcription, Gene Expression, Nested PCR, Sequencing, Expressing, Plasmid Preparation, Software, Microscopy

Journal:

Article Title: Cold shock and regulation of surface protein trafficking convey sensitization to inducers of stage differentiation in Trypanosoma brucei

doi: 10.1101/gad.323404

Figure Lengend Snippet: Subcellular localization of EP1:GFP in bloodstream-stage T. brucei MITat1.2. All images represent a single confocal plane extracted from digitally deconvolved multichannel 3D image data sets that were acquired using the same batch of fixed and permeabilized cells expressing EP1:GFP from the VSG221 expression site (pES221.EG). The left column images show an intensity merge of green EP1:GFP and blue DAPI fluorescence. The middle column shows the intracellular localization of markers, and the right column images show a merge of the three channels. (A) Cell surface staining by rabbit polyclonal VSG221 antiserum (1:1000) (red). (B) Endogenous EP (and EP1:GFP) detected by a mouse monoclonal EP antibody (1:500) (red). (C) The ER chaperone BiP detected by polyclonal rabbit anti-BiP (antibody courtesy of Jay Bangs, Madison; 1:500). (D) The Golgi marker TbRAB31 detected by rabbit anti-TbRAB31 (antibody courtesy of Mark Field, London; 1:200). (E) Endocytosed Alexa Fluor 594 conjugated dextran (see Materials and Methods). (F) Endocytosed biotinylated VSG (see Materials and Methods) detected with Alexa Fluor 594-conjugated streptavidin (Molecular Probes). (G) The lysosome visualized with a mouse monoclonal antibody directed against the lysosomal membrane protein p67 (antibody courtesy of David Alexander and Jay Bangs, Wisconsin; 1:1000). Alexa Fluor 594-conjugated goat anti-rabbit second antibody (1:2000) (A,C,D) or CY3-conjugated sheep anti-mouse second antibody (1:1000) (B,G) was used.

Article Snippet: The middle column shows the intracellular localization of markers, and the right column images show a merge of the three channels. ( A ) Cell surface staining by rabbit polyclonal VSG221 antiserum (1:1000) (red). ( B ) Endogenous EP (and EP1:GFP) detected by a mouse monoclonal EP antibody (1:500) (red). ( C ) The ER chaperone BiP detected by polyclonal rabbit anti-BiP (antibody courtesy of Jay Bangs, Madison; 1:500). ( D ) The Golgi marker TbRAB31 detected by rabbit anti-TbRAB31 (antibody courtesy of Mark Field, London; 1:200). ( E ) Endocytosed Alexa Fluor 594 conjugated dextran (see Materials and Methods). ( F ) Endocytosed biotinylated VSG (see Materials and Methods) detected with Alexa Fluor 594-conjugated streptavidin (Molecular Probes). ( G ) The lysosome visualized with a mouse monoclonal antibody directed against the lysosomal membrane protein p67 (antibody courtesy of David Alexander and Jay Bangs, Wisconsin; 1:1000).

Techniques: Expressing, Fluorescence, Staining, Marker